When a city bank mysteriously vanishes into the street, J. Jonah Jameson sends a reporter named Hammond to investigate, while sending Peter to visit a scientist who has recorded voices supposedly from the center of the earth. Peter visits the scientist, and when a recording is played for him, he discovers it is the men, or beasts, who made the bank disappear, and they are plotting another one. Realizing it is only a few minutes until the plot is carried out, Peter becomes Spidey & heads for the bank. Sure enough, the bank disappears into the earth! Spidey follows it down & finds a strange kingdom of creatures, the Mole-Men. When the leader announces a plan to subjugate the people who were in the building when it was taken, Spidey moves into action & overpowers the Mole-Men, and both they & their hostages will be in for a surprise.
The Sky-Master plots to wreak havoc on New York from his dirigible, so Spider-Man hitches a ride to burst his balloon.
A new nuclear reactor has been built in the heart of Manhattan, and Peter Parker's science class is researching it. Peter is asked by his attractive classmate, Sonya, to help her that night with research about the reactor. As Spidey is making his way to Sonya's home, trouble is brewing. A demented radiation specialist breaks into the new reactor and threatens the city with ransom; unless he is paid $10 million, given amnesty from prosecution, and given permission to build his own reactor, he will use anti-gravity rays on Manhattan to lift it into the sky. When his demands are refused, he does exactly that, and makes a ransom demand of $100-million, along with the other conditions, and unless his demands are met he will deactivate the ray & send Manhattan plummeting back to earth. Spidey must abort his date with Sonya & tunnel into the reactor to stop the madman. The specialist, who has speculated Spidey's powers came from radiation, tries to neutralize them by subjecting Spidey to anti-radiation. Unfortunately for him, Spidey resists the anti-radiation by force of will & kicks the specialist in the face. The stunned specialist fires his gun at Spidey, but misses, and the ray from the gun hits the controls sustaining the anti-gravity ray. As Manhattan starts to drop like a stone, Spidey must neutralize the psychotic specialist quickly.
Peter Parker overhears talk of a laboratory producing imitation pharmaceuticals, investigation as Spider-Man lead him to find out the whole plot has been engineered by a rotund mobster called the Kingpin.
Meek Peter Parker is bitten by a radioactive spider and acquires super-powers. He decides to use his new powers to get rich, but when tragedy strikes close to home, he learns a valuable lesson and vows to fight crime instead.
The Flys, a.k.a. the Patterson Twins, are paroled and freed. However, Spider-Man does not believe this and for a reason: they are framing him for all the heists they've done. It's up to Spidey to catch them in their act.
Dr. Noah Boddy releases Electro, the Green Goblin and the Vulture to confront Spider-Man in a series of showdowns. Then, a Chameleon-like criminal eludes police and Spider-Man alike.
Spider-Man is back in Florida, where he must find the secret behind an old Spanish Fort, guarded by some would-be Conquistadors.
Doc Ock steals a device in order to rule the world. Spider-Man must do something to keep this evil mastermind to achieve his dreadful threat.
In order to do a final battle with Spider-Man, the Vulture abducts J.Jonah Jameson and puts him next to a revolving pendulum. It's up to Spidey to stop the Vulture, otherwise his other nemesis Jamieson ends up in two halves.
Spider-Man must stop a couple of former clever circus acrobats, dressed in dark, specialized in robbing big bank safes.
The Rhino sets a gold standard for his crime spree, by creating a statue of himself. Then, a mastermind uses a cow-boy and a thug to find blueprints to destroy New York. It's up to Spider-Man to foil and neutralize their evil plan.
Trying to foil a mystery keeping the demolition of a playhouse, Spider-Man meets Blackwell the Magician who, behind his mischievous tricks, has a message to say to him.
A fakir is giving a hard time to Spider-Man, especially that he is a clever magician who can send spells to animals to attack the hero.
A series of heists are made by mythical creatures, ruled by a woman who holds a library. Spider-Man must put a stop to this before it goes too far.
Spider-Man must stop a magnetism wielding mad scientist. Spidey must rescue a foreign prime minister that only he knows is kidnapped by an imposter.
An exotic African idol is used to hypnotize Jameson into embezzling for a villain. A mysterious hooded figure is using robotic store mannequins to commit robberies.
A bad case of the common cold complicates Spider-Man's efforts to stop The Rhino from stealing the components of a secret weapon.
Escaped from jail, Electro is trying to find, then trap Spiderman for revenge.
The Green Goblin tries to make a pact with J.Jonah Jameson in order to catch Spider-Man. However, Spidey must beware of his foe's witching powers.
The Scorpion, a super-human creation designed to vanquish Spider-Man, has a lethal sting in his tail and he's ready to sting J. Jonah Jameson. The Sandman heists the priceless Goliath Diamond and Spider-Man is the fall guy for the crime.
Spider-Man fights the Vulture who is controlling a massive flock of birds. In the second segment, the Daily Bugle publisher hunts Spider-Man with a remote-controlled robot.
After framing Spider-Man, Mysterio offers to kill the superhero for J. Jonah Jameson for a fee.
Spider-Man faces Electro, a man capable to shoot lightning rods with his hand and traveling on electric lines to go to his next heist.
Out in the countryside, Peter escapes an accident with the help of his Spider-Man disguise, but discovers a cave, with Dr.Octopus in charge of some power machines due to rule the World. Doc Ock captures Spidey, then Miss Brant who happened to be in the area. The villain is keeping both prisoners as witnesses for his demonstration of power.